Circrna pulldown probe
WebFeb 14, 2024 · c Biotin-labeled sense or antisense circPUM1 probes were used for RNA-protein pull-down against KYSE30 cells lysates following western blot analysis of … WebFeb 26, 2024 · A biotin-labeled circRAB11FIP1 probe was provided by GenePharma. ... Then, qPCR was used to determine miRNA expression. CircRNA pulldown. The circRNA pulldown was performed as previously described ...
Circrna pulldown probe
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Web新一代circRNA pull-down技术路线. 本项技术在吉赛生物circRNA过表达载体技术基础上，将RNA标签体系引入circRNA中，实现在细胞内表达带有特异性标签的circRNA；通过RNA标签与捕获蛋白的高度特异且稳定的相 … WebApr 11, 2024 · Nuclear-cytoplasmic fractionation and FISH were used to identify the localization of circRNA and miRNA. Pull-down, RIP, and luciferase activity assays were performed to show the biding ability of circRNA, miRNA and target proteins. ... Target Prove is BaseScope Probe-BA-Hs-PALM-E7E2-circRNA (Advanced Cell Diagnostics China, …
WebSep 1, 2024 · To pull down the miRNA by circRNA, biotinylated-circSMARCC1 probe (5′-CATCTTCCTCATCACAGCAC-3′) was synthesized by RiboBio (Guangzhou, China), and the oligo probe (5′-TATGTTGTTGATTTGCTGGC-3′) was used as a control. CircRNA pulldown assay was carried out using PierceTM Magnetic RNA-Protein Pull-Down Kit … WebSep 22, 2024 · General workflow of the circular RNA pull-down assay The workflow includes probe template generation, amplification, and biotin-labeling process. Lysates …
Web(c-e) RNA-Pulldown was performed in PCa cells using hsa_-circ_0063329 and oligo probes. (f) Luciferase reporter assay was used to determine the effects of four predicted miRnas on the luciferase ... WebSep 9, 2024 · RNA pull down and mass spectrometry Biotin-labeled oligonucleotide probes targeting junction sites of circRNAs were synthesized by SunBio Biomedical Technology …
WebSep 9, 2024 · RNA pull down and mass spectrometry Biotin-labeled oligonucleotide probes targeting junction sites of circRNAs were synthesized by SunBio Biomedical Technology Co., Ltd (Shanghai, China)....
WebDec 7, 2024 · For circRNA pulldown experiments, a dedicated biotin-labeled circDOCK1 probe was used, and circDOCK1-related RNAs were purified prior to qRT–PCR. Based on the experimental results, compared with control group samples, samples pulled down with the circDOCK1-specific probe showed notable enrichment of only miR-339-3p (Fig. 3F). highland bsaWebCircRNA pull-down assay. A biotin-labeled circPTPRA probe was designed and synthesized by RiboBio Co., Ltd. The RNA pull-down assay was performed according to the instruction of the Pierce™ Magnetic RNA–Protein Pull-Down Kit (Thermo Scientific). The probe sequences used for the RNA pull-down assay are summarized in Additional … highland brown atlasWebJun 2, 2024 · A novel and highly specific circRNA-pulldown followed by mass spectrometry analysis identified 94 circFOXK2-interacting proteins, which were involved in cell adhesion, mRNA splicing, and structural molecule activity. Of these, circFOKX2 interactions with YBX1 and hnRNPK enhanced expression of oncogenes NUF2 and PDXK. how is bharat movie reviewhttp://www.circrna.com.cn/?p=4923 highland brow galgateWebJan 1, 2015 · After incubation of LOC100506178 probes with streptavidin-coated magnetic beads, ... The RNA pull-down assay was adapted from previously published studies [27] or protocols [28]. Unedited and ... how is bharatpe different from paytmWebFeb 14, 2024 · CircRNAs are a class of covalently closed cyclic RNA molecules, most of which are resistant to RNase R digestion and are generally produced by the back-splicing of linear pre-mRNA. 11, 12 CircRNAs... highland broxbourneWebAbnormal levels or dysregulation of miRNA or circRNA are linked to several cancerous pathologies, so that they are receiving a large attention as diagnostic and prognostic tools. ... and the Atto390 dye, bound to the 5′ end of miR4749-5p, provides the acceptor (A), with these probes representing a very appropriate D-A couple for FRET . The ... how is bf skinner theory used in schools